Bacterial starter cultures are fundamental for sausage fermentation. These lactic acid bacteria ferment the raw meat and guarantee that final product is acidic enough to kill pathogens that might have existed. University of Copenhagen and University Collage Cork have studied the effect of antibiotic residues, oxytetracycline, pencicillin and erythromycin, in the meat used for sausage production (Ingmer et al, mBio 2012 vol.3). Data show that residual antibiotics in the meat can prevent or reduce fermentation by the lactic acid bacteria, affecting the quality of the final product. Antibiotics are frequently used in veterinary medicine both for therapeutic value ant to enhance growth and food efficiency. Consequently, these practices might lead to a possible occurrence of residues in food. Food contaminated with antibiotic residues should be prevented from reaching the consumer. The detection of antibiotics is compulsory and levels should comply with the Maximum Residue Limits (MRL) state by the European Union (Commission Regulation (EU) No 37/2010). ZEU-INMUNOTEC, leader company in food quality, offers Explorer kit for detection of antibiotics en meat to avoid the presence of antibiotics in food chain. Source: mBiosphere
Laboratories from Spain, Croatia, Namibia or Neatherlands met last 14th -15th of June at the Marine Institute (Ireland) in a whorkshop about “Analysis of Marine Biotoxins”. Experts on the subject such as Dr. Arjen Gerssen, from The Netherlands NRL (National Reference Laboratory) or Dr.Begoña Ben-Gigirey, from the EURLMB (European Reference Laboratory for Marine Biotoxins) talked about PSP and lipophilic toxins testing. Dr. Elena Domínguez from ZEU-INMUNOTEC offered a lab demonstration on the phosphatase inhibition assay-OkaTest– for quantitative determination of OA-toxins in bivalve molluscs. Participants had the opportunity to see the simplicity of an assay that can be implemented for official OA testing, combined with LC-MS or HPLC, or end products according to the EC No. 15/2011. Other lab sessions on lipophilic and PSP toxins completed the OkaTest demonstration as part of this workshop. Oral presentations and lab demonstrations involved active participation of attendees. Lab experiences and procedures were shared, giving participants different views that will allow them improving their current methodologies.
The Food Safety and Standards Authority of India assessed a milk analysis over 33 states founding nearly 70% of milk samples were contaminated. The survey showed fraudulent manipulation since diluting milk in water from adding skimmed milk powder, glucose, hydrogen peroxide or detergent. In one hand, water addition reduces milk nutritional value and, even, can cause additional health problems if water is contaminated. On the other hand, milk adulterants detected produce hazardous health effects. Detergent can cause food poisoning. Other contaminants as urea or sodium carbonate cause gastroenteritis as immediate effect, but long term effects are more serious. India is not the only country where milk is adulterated; China, Peru and Ecuador also have been suffering these frauds. ZEU Inmunotec, a Spanish biotechnology company, believes that milk and its derivatives have high nutritional value. Therefore milk quality should be carefully controlled. To achieve this goal ZEU Inmunotec offers a wide variety of kits to perform different tests: screening of antibiotic in milk (Eclipse 3G, Eclipse Farm 3G, Eclipse 50 and EQUINOX), rapid inmunoenzimatic tests for identification of milk species (IC caprine, bovine and RC bovine, caprine) and inmunoenzimatic tests for detecting addition of calostrum to milk (Calokit-cow, sheep and goat). Fuentes: BBC News and The Food Safety and Standards Authority of India
Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins
Toxins. 2012, 4, 339-352; http://www.mdpi.com/2072-6651/4/5/339/pdf
A phosphatase inhibition assay for detection of okadaic acid (OA) toxins in shellfish, OkaTest, was single laboratory validated according to international recognized guideline(AOAC, EURACHEM). Special emphasis was placed on the ruggedness of the method and stability of the components. All reagents were stable for more than 6 months and the method was highly robust under normal laboratory conditions. The limit of detection and quantification were 44 and 56 μg/kg, respectively; both below the European legal limit of 160 μg/kg. The repeatability was evaluated with 2 naturally contaminated samples. The relative standard deviation (RSD) calculated was 1.4% at a level of 276 μg/kg and 3.9% at 124 μg/kg. Intermediate precision was estimated by testing 10 different samples (mussel and scallop) on three different days and ranged between 2.4 and 9.5%. The IC50 values of the phosphatase used in this assay were determined for OA (1.2 nM), DTX-1 (1.6 nM) and DTX-2 (1.2 nM). The accuracy of the method was estimated by recovery testing for OA (mussel, 78–101%; king scallop, 98–114%), DTX-1 (king scallop, 79–102%) and DTX-2 (king scallop, 93%). Finally, the method was qualitatively compared to the mouse bioassay and LC-MS/MS.
OkaTest, based on a phosphatase inbhibition assay and manufactured by ZEU-INMUNOTEC, fulfils the Regulations (2074/2005 and 15/2011) requirements and can be used for detection of Okadaic Acid group toxins (OA, DTX1,DTX2, DTX3 including their esters). http://www.aesan.msps.es/CRLMB/web/otros_procedimientos/other_crlmb_standard_operating_procedures.shtml