The Food Safety and Standards Authority of India assessed a milk analysis over 33 states founding nearly 70% of milk samples were contaminated. The survey showed fraudulent manipulation since diluting milk in water from adding skimmed milk powder, glucose, hydrogen peroxide or detergent. In one hand, water addition reduces milk nutritional value and, even, can cause additional health problems if water is contaminated. On the other hand, milk adulterants detected produce hazardous health effects. Detergent can cause food poisoning. Other contaminants as urea or sodium carbonate cause gastroenteritis as immediate effect, but long term effects are more serious. India is not the only country where milk is adulterated; China, Peru and Ecuador also have been suffering these frauds. ZEU Inmunotec, a Spanish biotechnology company, believes that milk and its derivatives have high nutritional value. Therefore milk quality should be carefully controlled. To achieve this goal ZEU Inmunotec offers a wide variety of kits to perform different tests: screening of antibiotic in milk (Eclipse 3G, Eclipse Farm 3G, Eclipse 50 and EQUINOX), rapid inmunoenzimatic tests for identification of milk species (IC caprine, bovine and RC bovine, caprine) and inmunoenzimatic tests for detecting addition of calostrum to milk (Calokit-cow, sheep and goat). Fuentes: BBC News and The Food Safety and Standards Authority of India
Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins
Toxins. 2012, 4, 339-352; http://www.mdpi.com/2072-6651/4/5/339/pdf
A phosphatase inhibition assay for detection of okadaic acid (OA) toxins in shellfish, OkaTest, was single laboratory validated according to international recognized guideline(AOAC, EURACHEM). Special emphasis was placed on the ruggedness of the method and stability of the components. All reagents were stable for more than 6 months and the method was highly robust under normal laboratory conditions. The limit of detection and quantification were 44 and 56 μg/kg, respectively; both below the European legal limit of 160 μg/kg. The repeatability was evaluated with 2 naturally contaminated samples. The relative standard deviation (RSD) calculated was 1.4% at a level of 276 μg/kg and 3.9% at 124 μg/kg. Intermediate precision was estimated by testing 10 different samples (mussel and scallop) on three different days and ranged between 2.4 and 9.5%. The IC50 values of the phosphatase used in this assay were determined for OA (1.2 nM), DTX-1 (1.6 nM) and DTX-2 (1.2 nM). The accuracy of the method was estimated by recovery testing for OA (mussel, 78–101%; king scallop, 98–114%), DTX-1 (king scallop, 79–102%) and DTX-2 (king scallop, 93%). Finally, the method was qualitatively compared to the mouse bioassay and LC-MS/MS.
OkaTest, based on a phosphatase inbhibition assay and manufactured by ZEU-INMUNOTEC, fulfils the Regulations (2074/2005 and 15/2011) requirements and can be used for detection of Okadaic Acid group toxins (OA, DTX1,DTX2, DTX3 including their esters).
PP2A (protein phosphatase) technique appears satisfactory for the analysis of DSP toxins in shellfish and is recommended as a rapid assay method for screening and end product testing. This is one of the conclusions raised from a study carried out within the water project http://www.nppwater.com/index.php Mussel samples from the biotoxins monitoring program carried by the Irish Marine Institute, Galway, were analysed. Samples were extracted from 3 sites (inner, middle and outer) from Killary Harbour, Ireland, in 2009 and 2010. Mussels from a DSP event were tested by Mouse Bioassay (MBA), ELISA (Abraxis), LC-MS and protein phosphatase PP2A assay (OkaTest). ELISA kits showed matrix effects, but PP2A technique (OkaTest, ZEU-INMUNOTEC) was recommended as rapid assay. More information on http://www.nppwater.com/data_access.php?id=85
The MicroCystest plate kit has been recently evaluated within the Environmental Technology Verification (ETV) Program. The E.P.A. supports the ETV program to further environmental protection by accelerating the acceptance and use of improved and cost effective technologies. Microcystest kit is a rapid enzymatic test for detection of microcystins in drinking and recreational water. Microcystins are a group of hepatotoxins produced by cyanobacteria during and after blooms in water bodies, as a result of eutrophication. They are considered the most harmful cyanotoxins due to their natural abundant and potent toxicity. Incidences of animal and human fatalities caused by microcystins have led the World Health Organization to introduce a provisional upper limit of 1 µg/L in drinking water. The toxicity of microcystins is associated with the inhibition of protein phosphatases 1 and 2A enzymes, which can lead to hepatocyte dysfunction. MicroCystest kit uses this inhibitory reaction for determination of the microcystins concentration by means of a microtiter plate and a colorimetric substrate. The enzyme hydrolyses the substrate and the product can be determined by an absorbance measurement at 405 nm. As the ability of the PP to hydrolyse the substrate depends on the presence of microcystins and analogues in the samples, the toxin concentration can be calculated by using a standard curve. MicroCystest is the first and only commercially available kit based on the inhibition of the PP2A activity by microcystins, and therefore able to detect all potential toxic microcystins present in the sample (over 80 different varieties of MCs have been described up-to-day).