Aflatoxin M1 ELISA

Quantify the content of aflatoxin M1 in milk and dairy products in your laboratory using an easy ELISA assay.

 

Do you need to know the quantity of aflatoxin M1 in your products?
Do you have many products to test and you need quick results?
Do you need to detect very low levels of aflatoxin M1 in food with special requirements?

 

EUROPROXIMA kits only avaliable for sale in SPAIN

 

HOW DOES IT HELP YOU?

 

ELISA tests for aflatoxins M1 help you processing a high number of samples with good reproducibility and high sensitivity, complying with the specifications required by the UE in milk and milk products.

MATRIX
MILK

DAIRY PRODUCTS

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Sample extraction:

Extract with organic solvents.

2. Assay:

Add standards/samples and reagents provided in the kit.

(2 / 3 h depends on the test type )

3. Result:

Photometrical reading at 450 nm

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Resultados:

Lectura fotométrica a 450nm

TEST PRINCIPLE

ELISA tests for detection of aflatoxin M1 are based on competition between aflatoxins contained in the sample and aflatoxins conjugated with peroxidase by joining to anti-aflatoxin antibodies to make antigen-antibody complexes. These complexes are detected by adding a chromogenic substrate, which reacts with peroxidase, making colour.

TECHNICAL DATA

  • Application: Quantification of aflatoxin M1 in milk and milk products.
  • Test time: 2 to 3. 5 h (depending on reference)
  • Detection limits: 2. 3 – 5 ppt (depending on matrix and kit reference)

 

Validations:

Europroxima ELISA tests are internally validated according to European Decision 2002/657/EC.

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