Eclipse 100

Detects more than 50 antibiotics in milk in a single assay in a large number of samples.

 

Are you worried about ruining your production of fermented products due to the presence of antibiotics in milk?
Are you worried about complying with the maximum residue limits established by the European legislation?

 

HOW DOES IT HELP YOU?

 

Eclipse100 helps you to screen more than 50 antibiotics from 8 groups in just one assay, thus avoiding problems in the production of fermented dairy products, as well as to comply with the maximum residue limits in milk established by the European Union.
Eclipse100 allows you to analyse from just one sample to a high number of samples in approximately 3.5 hours. Results can be read visually or using a photometer for an objective reading of the results.

MATRIX
RAW MILK

MILK POWDER

HEAT-TREATED MILK

COW, GOAT AND SHEEP RAW MILK

APPLICATIONS AND USERS
PROCESSING PLANT

LABORATORY

ASSAY PROCEDURE

1. Add sample:

50μL of milk

2. Incubation:

Diffuse 1h at room temp. and wash with distilled water

Incubate at 65ºC

3. Results:

Visual or photometric reading at 595nm and 650nm

TEST PRINCIPLE

ECLIPSE test is based on the growth inhibition of the Geobacillus stearothermophilus bacterium. In a specific culture medium and under suitable conditions, the spores of this bacterium germinate and multiply, acidifying the medium, producing a colour change in the culture medium from blue to yellow. If the milk sample contains an antimicrobial concentration higher than the test detection limit, the bacterial growth is inhibited and therefore the colour change of the medium will not occur.

TECHNICAL DATA

  • Scope: detection of more than 50 antibiotics in milk from the beta-lactams, tetracyclines, sulphonamides, macrolides, aminoglycosides, lincosamides, anasamycins and sulfones groups
  • Assay time: two steps: 1 h diffusion + 2h 30 min at 65 °C
  • Results: visual or photometric
  • Format: whole microtiter plate of 96 wells

 

Validations:

Internal validation: According to ISO 13969:2003.
External validation by the Belgian Reference Laboratory ILVO.

 

Legislation:

Commission Regulation (EU) No 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin.

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