Domotest

Quantifies domoic acid in bivalve molluscs in an easy lab assay.

 

Do you need to assure that your product will not cause amnesic poisoning syndrome (ASP)?
Do you need to demonstrate that your molluscs comply with the legal requirements?
Would you like to know when is the best time to harvest or sell your molluscs?

 

HOW DOES IT HELP YOU?

 

Domotest can help you to choose the best area and time to harvest, preventing extraction of contaminated molluscs from areas near those ones closed due to presence of these toxins.
Performing shelf-control with Domotest before harvesting or as end-product testing you can assure molluscs are fit for consumption and avoid unnecessary process contaminated products.

En caso de ser un laboratorio de análisis podrás ofrecer resultados en un plazo de tiempo muy corto y optimizar costes, ya que Domotest permite analizar hasta 41 muestras en aproximadamente 1h.

MATRIX
MOLLUSCS

MUSSELS

CLAMS

OYSTER AND SCALLOPS

APPLICATIONS AND USERS
SHELLFISH GROWERS AND HARVESTERS

PROCESSORS

LABORATORY

ASSAY PROCEDURE

1. Samples/Standards:

+ Conjugate.
+ Antibody.
Incubation: RT/30min.

2. Add substrate:

RT/15 min.

3. Results:

Read at 450nm.

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Results:

Lectura fotométrica a 450nm

TEST PRINCIPLE

Domotest is an ELISA based on the competition of domoic acid (DA) extracted from the sample, and domoic acid labelled with peroxidase to bind specific anti-domoic acid antibodies to form antigen-antibody complexes.

TECHNICAL DATA

  • Working range: 4-80 mg/Kg
  • Assay time: 15 min extraction. 30 + 15 min assay
  • Presentation: 96-well microtiter plate. Total of 41 samples
  • Equipment: Microtiter plate reader at 450 nm

 

Validations:

Internal validation.

 

Legislation:

Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin.

Commission Regulation (EC) No 2074/2005 of 5 December 2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004 of the European Parliament and of the Council and for the organisation of official controls under Regulation (EC) No 854/2004 of the European Parliament and of the Council and Regulation (EC) No 882/2004 of the European Parliament and of the Council, derogating from Regulation (EC) No 852/2004 of the European Parliament and of the Council and amending Regulations (EC) No 853/2004 and (EC) No 854/2004.

 

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