Saxitest

Simple laboratory assay for screening of saxitoxins in bivalve molluscs and water.

 

Do you need to assure that your product will not cause paralytic poisoning syndrome (PSP)?
Do you need to demonstrate that your molluscs comply with the legal requirements?
Would you like to know when is the best time to harvest or sell your molluscs?

 

HOW DOES IT HELP YOU?

 

Saxitest can help you to choose the best area and time to harvest, preventing extraction of contaminated molluscs from areas near those ones closed due to presence of these toxins.
Performing shelf-control with Saxitest before harvesting or as end-product testing you can assure molluscs are fit for consumption and avoid unnecessary process contaminated products.
En caso de ser un laboratorio de servicios podrás ofrecer resultados en un plazo de tiempo muy corto y optimizar tus costes ya que Saxitest permite analizar hasta 40 muestras en aproximadamente 1h.

MATRIX
BIVALVE MOLLUSCS

MUSSELS

CLAMS

OYSTER AND SCALLOPS

APPLICATIONS AND USERS
SHELLFISH GROWERS AND HARVESTERS

PROCESSORS

LABORATORY

ASSAY PROCEDURE

1. Samples/Standards:

+ Conjugate.
+ Antibody.
Incubation: RT/30min.

2. Add substrate:

RT/15 min.

3. Results:

Read at 450nm

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Results:

Lectura fotométrica a 450nm

TEST PRINCIPLE

SaxiTest is an ELISA based on the competition saxitoxins extracted from the sample with saxitoxins labelled with peroxidase to bind specific anti-saxitoxin antibodies to form antigen-antibody complexes.

TECHNICAL DATA

  • Working range: 112.5-2700 µg/Kg
  • Assay time: 15 min extraction. 30 + 15 min assay
  • Presentation: 96-well microtiter plate. Total of 41 samples.
  • Equipment: Microtiter plate reader at 450 nm

 

Validations:

Validación interna siguiendo guías Eurachem.

 

Legislation:

Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin.

Commission Regulation (EC) No 2074/2005 of 5 December 2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004 of the European Parliament and of the Council and for the organisation of official controls under Regulation (EC) No 854/2004 of the European Parliament and of the Council and Regulation (EC) No 882/2004 of the European Parliament and of the Council, derogating from Regulation (EC) No 852/2004 of the European Parliament and of the Council and amending Regulations (EC) No 853/2004 and (EC) No 854/2004.

Commission Regulation (EU) No 15/2011 of 10 January 2011 amending Regulation (EC) No 2074/2005 as regards recognised testing methods for detecting marine biotoxins in live bivalve molluscs.

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