Are you considering of doing allergen control but you do not know how to approach it?
Do you want to verify cleaning between productions when sharing production lines?
Do you have many products to analyse and you need results immediately or within a day?
Are you implementing an IFS, BRC, ISO 22000 2005 quality standard?
Do you work with powdered egg and you could have contaminations after cleaning?
The speed and simplicity of the Proteon Express tests allow adapting the allergen analysis to the time and place you need. Having specific information will allow us to make immediate decisions between productions, detect insufficient cleanings, control critical points of contamination and ensure the release of your product to the market without risk.
Immunochromatographic assay, in a fast strip format, based on the detection of ovalbumin by a specific antibody in a non-competitive sandwich assay.
Validations:
Internal validation following International guidelines:
Appendix M: Validation Procedures for Quantitative. Food Allergen ELISA Methods: Community Guidance and Best Practices. AOAC 2012 (http://www.eoma.aoac.org/app_m.pdf).
Appendix F: Guidelines for Standard Method Performance Requirements. Official Methods of Analysis (2016), AOAC INTERNATIONAL, Rockville, MD, USA (http://www.eoma.aoac.org/app_f.pdf)
Standard Method Performance Requirements (SMPRs®) for Quantitation of Chicken Egg by ELISA-Based Methods. AOAC (SMPRs®) 2017.020 (http://members.aoac.org/aoac_prod_imis/AOAC_Docs/ISPAM/SMPR_2017.020.pdf)
Standard Method Performance Requirements (SMPRs®) for Detection and Quantitation of Selected Food Allergens. AOAC SMPR 2016.002. AOAC INTERNATIONAL, Rockville, MD, USA (https://www.aoac.org/aoac_prod_imis/AOAC_Docs/SMPRs/SMPR%202016_002.pdf).
Galan-Malo et al. (2017) Detection of egg and milk residues on working surfaces by ELISA and lateral flow immunoassays test. Food Control 74: 45-53.
Supporting bibliography for validation of antibodies and extraction methods of food samples:
De Luis et al. (2008) Evaluation of indirect competitive and double antibody sandwich ELISA tests to determine β-lactoglobulin and ovomucoid in model processed foods. Food and Agricultural Immunology, 19: 339-350.
Legislation:
Regulation (EU) No 1169/2011 of the European Parliament and of the Council of 25 October 2011 on the provision of food information to consumers, amending Regulations (EC) No 1924/2006 and (EC) No 1925/2006 of the European Parliament and of the Council, and repealing Commission Directive 87/250/EEC, Council Directive 90/496/EEC, Commission Directive 1999/10/EC, Directive 2000/13/EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission Regulation (EC) No 608/2004 Text with EEA relevance.
