Acuvet Turbovet Bovine Hp

Quantify bovine haptoglobin automatically, with high precision and in a few minutes: all the advantages of a turbidimetric method with the reliability of a species-specific test that is not affected by hemolysis. Testing haptoglobin you will be able to early detect inflammatory or infectious diseases, assess the level of stress of the animal and obtain a quantitative measure of animal welfare.

Do you want to determine if the animals arrive to the farm in optimal conditions or have suffered from excessive stress during transport which can increase the incidence of respiratory disease?
Do you want to have a quantitative indicator of animal health and welfare that is objective and easy to determine?
Do you want to detect subclinical disease or evaluate if the treatments applied to the animal are effective?
Do you want to quantify with confidence haptoglobin using a fast and automated method and without worrying about whether the samples are hemolyzed?

 

HOW DOES IT HELP YOU?

 

Haptoglobin is used in the diagnosis and monitoring of infectious or inflammatory diseases in cattle, and provides a quantitative and objective measure of the animal’s health and welfare status. It helps to detect diseases such as metritis, mastitis or bovine respiratory disease early, and to evaluate if the applied treatments are effective. With the Turbovet method the analysis can be automated and the results are obtained in a few minutes. Furthermore, it provides sufficient sensitivity to measure basal levels, high precision and reproducibility, and unlike the colorimetric method, it does not present interference by hemolysis. Thus it is an ideal tool for production quality control systems or animal welfare certification programs, as well as for the clinical diagnostic laboratory, which can easily incorporate this parameter into their analytical profiles.

MATRIX
SERUM & PLASMA

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Load sample:

Insert samples into the analyzer.

2. Assay:

Addition of R1, sample and R2, and first reading.

Incubation for 5 minutes and second reading.

3. Result:

Read results on the analyzer.

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Resultados:

Photometrical reading at 450nm

TEST PRINCIPLE

Particle-enhanced turbidimetric method: in the reaction media haptoglobin from serum reacts with specific antibodies fixed to latex particles, forming aggregates that originate and increase of turbidity.  The increase of turbidity is determined with a photometric measure, and is proportional to the concentration of haptoglobin in the sample. The assay is calibrated according to the European Reference Serum for Bovine Acute Phase Proteins (European Concerted Action QLRT-1999-01532).

TECHNICAL DATA

  • Specific for bovine
  • Limit of detection: 5 mg/L
  • Working range:  0-400mg/L
  • Time of assay: 5 minutes
  • Reagents: ready to use and very stable
  • Automatic: adaptable to any open clinical chemistry analyzer.
  • No interferences by hemoglobin (20g/L), bilirubin (0.15mg/L), or triglycerides (10g/L)

 

Validations:

Validated by the Autonomous University of Barcelona, following CLSI and SECQ guidelines.

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