Turbovet Bovine ITIH4

Quantify bovine ITIH4 automatically, with high precision and in a few minutes: all the advantages of a turbidimetric method with the reliability of a species-specific test. Testing ITIH4 you will be able to detect problems of health and poor welfare in the animal with high sensitivity.

 

Do you want to have a better control of the on-farm health and welfare status?
Do you want to determine bovine ITIH4 with confidence?
Do you want to automate the assay with a clinical chemistry analyzer and have a rapid, robust and very robust method?

 

HOW DOES IT HELP YOU?

 

Using this biomarker in combination with haptoglobin allows detecting with high sensitivity problems of health or poor welfare in the herd. Turbovet bovine ITIH4 is the first method on the market for the analysis of ITIH4 by turbidimetry. With Turbovet bovine ITIH4 the results are obtained in a few minutes, the assay can be automated using a clinical chemistry analyzer, and a pre-dilution of the sample is not necessary. This reduces significantly the analyst’s working time, and consequently the costs. The test also has a very high precision and reproducibility, making it an ideal tool for production quality control systems or animal welfare certification programs.

MATRIX
SERUM & PLASMA

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Load sample:

Insert samples into the analyzer.

2. Assay:

Addition of R1 and sample, and first reading.

Addition of R2, incubation 5 min and second reading.

3. Results:

Read results on the analyzer.

Nombre del video

1. Sample dilution:

Aplicar muestra/patrón. Incubación.

2. Conjugate:

Incubation

3. Results:

Photometrical reading at 450nm

TEST PRINCIPLE

The test is based on immunoturbidimetry: ITIH4 from serum reacts with specific antibodies to form immunoaggregates, originating an increase of turbidity in the reaction media. The increase of turbidity is determined with a photometric measure, and is proportional to the concentration of ITIH4 in the sample. The assay uses a calibrator specific for swine. Calibrator´s values are assigned with an internal reference material, as no international standard for bovine ITIH4 exists.

TECHNICAL DATA

  • Specific for bovine
  • Working range: 0-4 mg/mL
  • Time of assay: 5 minutes
  • Reagents: Ready to use and very stable
  • Automatic: can be adapted to any open clinical chemistry analyzer.
  • No interferences by hemoglobin (20g/L), bilirubin (0.15g/L) or triglycerides (10g/L)

 

Validations:

Validated by the Autonomous University of Barcelona, following CLSI and SECQ guidelines.

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