Okatest

Quantifies all okadaic acid toxins and their associated toxicity.
Suplemmentary test to the reference method for quantification of okacaic acid toxins

 

Do you need to assure that your product will not cause diarrheic poisoning syndrome (DSP)?
Do you need to demonstrate that your molluscs comply with the legal requirements?
Would you like to know when is the best time to harvest or sell your molluscs?
¿Necesitas reducir costes o tiempo en el seguimiento en zonas cerradas por okadaico?

 

HOW DOES IT HELP YOU?

 

Okatest can help you to choose the best area and time to harvest, preventing extraction of contaminated molluscs from areas near those ones closed due to presence of these toxins.
Performing shelf-control with Okatest before harvesting or as end-product testing you can assure molluscs are fit for consumption and avoid unnecessary process of contaminated products.
Okatest te permite ofrecer resultados en el mismo día ya que puedes procesar hasta 43 muestras en unas 3 horas. Además, podrás combinarlo con el método de referencia obteniendo resultados para todo el grupo de toxinas lipofílicas en menos tiempo; así como hacer el seguimiento de las zonas cerradas por ácido okadaico.

OkaTest can therefore be used as a supplementary test to the reference method for determination of the OA-toxins group in molluscs according to the Commission Regulations (EC) No. 853/2004 and No. 15/2011 (http://www.aecosan.msssi.gob.es/en/CRLMB/web/public_documents/seccion/other_crlmb_standard_operating_procedures.htm)

MATRIX
BIVALVE MOLLUSCS

MUSSELS

CLAMS

OYSTER AND SCALLOPS

APPLICATIONS AND USERS
SHELLFISH GROWERS AND HARVESTERS

PROCESSORS

LABORATORY

ASSAY PROCEDURE

1. Samples extraction:

50μl sample/standard
70μl phosphatase.

2. Incubation:

30ºC/20 min.
90μl substrate – 30ºC/30 min.

3. Results:

Photometrical reading at 450 nm.

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Results:

Lectura fotométrica a 450nm

TEST PRINCIPLE

Inhibition of serine/threonine protein phosphatases (PP1 & PP2A) activity by this group of toxins is assumed to be responsible for these diarrheic disorders. Okatest based on the phophatase activity inhibition (PP2A) by OA-toxins group, responsible for diarrheic shellfish poisoning (DSP).
Phosphatase enzyme PP2A is able to hydrolyse a specific substrate, yielding a product that can be detected at 405 nm. Samples containing toxins from the okadaic acid group will inhibit the enzyme activity proportionally to the amount of toxin contained in the sample.

TECHNICAL DATA

  • Working range: 63 -352 μg OA equivalents/Kg.
  • Assay time: 1 h extraction plus 2 steps of 20 & 30 minutes.
  • Presentation: 96 and 48-well microtiter plate. Total of 43 samples.
  • Equipment: Microtiter plate reader at 405 nm.

 

Validations:

Intralaboratory validation:
Smienk et al. 2012 Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins. Toxins, 5, 339-352.

Inter-laboratory validadation:

Validación con 16 laboratorios procedentes de 11 países. Smienk et al. 2013. Quantitative Determination of the Okadaic Acid Toxins Group by a Colorimetric Phosphatase Inhibition Assay: Interlaboratory Study. Journal of AOAC International Vol. 96, No. 1.

Participation in interlaboratory studies: Quasimeme laboratory performance studies 2012-2015

 

 

Legislation:

Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin.

Commission Regulation (EC) No 2074/2005 of 5 December 2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004 of the European Parliament and of the Council and for the organisation of official controls under Regulation (EC) No 854/2004 of the European Parliament and of the Council and Regulation (EC) No 882/2004 of the European Parliament and of the Council, derogating from Regulation (EC) No 852/2004 of the European Parliament and of the Council and amending Regulations (EC) No 853/2004 and (EC) No 854/2004.

Commission Regulation (EU) No 15/2011 of 10 January 2011 amending Regulation (EC) No 2074/2005 as regards recognised testing methods for detecting marine biotoxins in live bivalve molluscs.

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