- Working range: 63 -352 μg OA equivalents/Kg.
- Assay time: 1 h extraction plus 2 steps of 20 & 30 minutes.
- Presentation: 96 and 48-well microtiter plate. Total of 43 samples.
- Equipment: Microtiter plate reader at 405 nm.
Smienk et al. 2012 Single Laboratory Validation of A Ready-to-Use Phosphatase Inhibition Assay for Detection of Okadaic Acid Toxins. Toxins, 5, 339-352.
Validación con 16 laboratorios procedentes de 11 países. Smienk et al. 2013. Quantitative Determination of the Okadaic Acid Toxins Group by a Colorimetric Phosphatase Inhibition Assay: Interlaboratory Study. Journal of AOAC International Vol. 96, No. 1.
Participation in interlaboratory studies: Quasimeme laboratory performance studies 2012-2015
OkaTest can therefore be used as a supplementary test to the reference method for determination of the OA-toxins group in molluscs according to the Commission Regulations (EC) No. 853/2004 and No. 15/2011 (http://www.aecosan.msssi.gob.es/en/CRLMB/web/public_documents/seccion/other_crlmb_standard_operating_procedures.htm)
Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin.
Commission Regulation (EC) No 2074/2005 of 5 December 2005 laying down implementing measures for certain products under Regulation (EC) No 853/2004 of the European Parliament and of the Council and for the organisation of official controls under Regulation (EC) No 854/2004 of the European Parliament and of the Council and Regulation (EC) No 882/2004 of the European Parliament and of the Council, derogating from Regulation (EC) No 852/2004 of the European Parliament and of the Council and amending Regulations (EC) No 853/2004 and (EC) No 854/2004.
Commission Regulation (EU) No 15/2011 of 10 January 2011 amending Regulation (EC) No 2074/2005 as regards recognised testing methods for detecting marine biotoxins in live bivalve molluscs.