ELISA Hp Bovine

Detect problems of health and poor welfare in your animals with high sensitivity testing bovine haptoglobin. Acuvet ELISA bovine Hp is a species-specific, sensitive and reliable test to determine this biomarker using basic equipment (ELISA reader).

 

Do you want to assess the on-farm animal health and welfare status?
Do you want to detect subclinical infections?
Do you want to quantify with confidence bovine haptoglobin without worrying about whether the samples are hemolyzed?
Do you want a sensitive method to analyze this biomarker in milk?

 

HOW DOES IT HELP YOU?

 

Haptoglobin is used in the diagnosis and monitoring of infectious or inflammatory diseases in cattle. It provides a quantitative and objective measure of the animal’s health and welfare status. It helps to detect early diseases such as metritis, mastitis or bovine respiratory disease, and to evaluate if the applied treatments are effective. Acuvet ELISA is a species-specific, sensitive and reliable test. It has not interference by hemoglobin as occurs with other haptoglobin assays, in particular the colorimetric method. It allows measuring haptoglobin not only in serum but in milk, where its increase is indicative of mastitis.

MATRIX
PLASMA

MILK

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Sample:

Dilute sample/pattern and apply

Room temp. incubation

2. Conjugate:

Wash and apply conjugated.

Incubation at room temperature.

Wash and add substrate.

3. Result:

Photometrical reading at 450nm

Nombre del video

1. Dilución de la muestra:

Apply sample/pattern. Incubation.

2. Conjugate:

Incubation

3. Results:

Photometrical reading at 450nm

PRINCIPIO DEL TEST

Acuvet ELISA bovine Hp is a sandwich-type ELISA. It uses antibodies and standard specific for the bovine Hp. The assay is calibrated according to the Reference Serum for Bovine Acute Phase Proteins (European Concerted Action QLRT-1999-01532).

TECHNICAL DATA

  • Specific for bovine
  • Limit of detection: 8 ng/mL
  • Working range: 10-300 ng/mL
  • Time of assay: 90 minutes

 

Validations:

Internal validation according to Eurachem´s guidelines

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