ELISA Pig HP

Asses the health status of your farms testing pig haptoglobin. Acuvet ELISA pig Hp is a pig-specific, sensitive and reliable test to determine this biomarker using basic equipment (ELISA reader).

 

Do you want to assess the on-farm health status?
Do you want to detect subclinical infections?
Do you want a tool that helps you to test the effectiveness of your biosecurity plans?
Do you want to quantify with confidence pig haptoglobin without worrying about whether the samples are hemolyzed?

 

HOW DOES IT HELP YOU?

 

Testing haptoglobin you can obtain a measure of the health status and hygienic conditions of your farms, as haptoglobin concentration increases in response to all kinds of infections. Furthermore, the presence of abnormally low values may be indicative of hemolytic processes since haptoglobin binds to free hemoglobin, forming complexes that will be removed from circulation. Acuvet ELISA is a reliable, species-specific test that has been designed to be used with hemolyzed samples. It does not have interference by hemoglobin as occurs with other haptoglobin assays, in particular the colorimetric method based on haptoglobin-hemoglobin binding.

MATRIX
SERUM & PLASMA

APPLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Sample:

Dilute sample/pattern and apply

Room Temperature Incubation

2. Conjugate:

Wash and apply conjugated.

Incubation at room temperature.

Wash and add substrate.

3. Result:

Photometrical reading at 450nm

Nombre del video

1. Sample dilution:

Aplicar muestra/patrón. Incubación.

2. Conjugate:

Incubation

3. Results:

Photometrical reading at 450nm

TEST PRINCIPLE

Acuvet ELISA pig Hp is a sandwich-type ELISA, based on polyclonal antibodies specific for pig haptoglobin. The assay is calibrated according to the European Reference Serum for pig Acute Phase Proteins (EU concerted Action QLK5-CT-1999-0153)

TECHNICAL DATA

  • Specific for swine
  • Limit of detection: 2 ng/mL
  • Working range: 10-280 ng/mL
  • Time of assay: 90 minutes

 

Validationss:

Internal validation according to Eurachem´s guidelines

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