Aflatoxin Total ELISA

Quantifies the content of total aflatoxins using an easy ELISA in a wide range of matrices.

 

Do you need to know the total content of aflatoxins (B1, B2, G1 and G2) in your products?
Do you have many products to test and you need quick results?

 

EUROPROXIMA kits only avaliable for sale in SPAIN

 

HOW DOES IT HELP YOU?

 

ELISA test for total aflatoxins helps you to process a high number of samples with good reproducibility, complying with the specifications required by the UE.

MATRIX
CEREALS

EGG

LIVER

SERUM

HONEY

RED PEPPER

APLICATIONS AND USERS
LABORATORY

ASSAY PROCEDURE

1. Sample extraction:

Extract with organic solvents.

2. Performing the assay:

Sequentially add standards/samples and the reagents provided in the kit.

(2 hours)

3. Result:

Photometrical reading at 450 nm

Nombre del video

1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Resultados:

Photometrical reading at 450 nm

TEST PRINCIPLE

ELISA tests for detection of total aflatoxins are based on competition between aflatoxins contained in the sample and aflatoxins conjugated with peroxidase by joining to anti-aflatoxin antibodies to make antigen-antibody complexes. These complexes are detected by adding a chromogenic substrate, which reacts with peroxidase, making colour.

TECHNICAL DATA

  • Application: quantification of aflatoxin B1 in food matrices.
  • Assay Time: 2. 5 – 3 hours
  • Detection Limits: 0. 2 – 1 ppb or 0. 03 – 2. 5 ppb depending on kit reference matrix

 

Validations:

Europroxima ELISA tests are internally validated according to European Decision 2002/657/EC.

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