Proteon Soja Express

In 10 minutes and without equipment, anyone can verify that your production is free from soy.

 

Are you considering of doing allergen control but you do not know how to approach it?
Do you want to verify cleaning between productions when sharing production lines?
Do you have many products to analyse and you need results immediately or within a day?
Are you implementing IFS, BRC or ISO 22000 2005 quality standard?
Are your products thermally processed and this can affect the detection of soy?

 

HOW DOES IT HELP YOU?

 

The speed and simplicity of the Proteon Express tests allow adapting the allergen analysis to the time and place you need. Having specific information will allow us to make immediate decisions between productions, detect insufficient cleanings, control critical points of contamination and ensure the release of your product to the market without risk.
Proteon Soja Express helps you to detect soy in raw or thermally processed (pasteurized and baked) products.

MATRIX
WORKING SURFACES

FOOD

RINSE WATER

APPLICATIONS AND USERS
FOOD INDUSTRY

CONSULTANTS

AUDITORS

INSPECTORS

LABORATORY

ASSAY PROCEDURE

1. Sample extraction:

Food or Surface.

2. Incubation:

Insert the strip and wait 10 min.

3. Results:

Visual reading.

TEST PRINCIPLE

Immunochromatographic assay, in a fast strip format, based on the detection of β-conglicinin by a specific antibody in a non-competitive sandwich assay. β-conglicinin is a protein thermally stable and constitutes the 30 % of the proteins of the seed. Moreover, it has been reported that it is able to cause severe allergic reactions.

TECHNICAL DATA

  • Limit of detection: 0.7 ppm of soy proteins.
  • Limit of detection in surfaces: 0.7 µg of soy proteins in 100cm2.
  • Assay time: 10 min.

 

Validations:

Internal validation following International guidelines:

Appendix M: Validation Procedures for Quantitative. Food Allergen ELISA Methods: Community Guidance and Best Practices. AOAC 2012 (http://www.eoma.aoac.org/app_m.pdf).

Appendix F: Guidelines for Standard Method Performance Requirements. Official Methods of Analysis (2016), AOAC INTERNATIONAL, Rockville, MD, USA (http://www.eoma.aoac.org/app_f.pdf)

Standard Method Performance Requirements (SMPRs®) for Detection and Quantitation of Selected Food Allergens. AOAC SMPR 2016.002. AOAC INTERNATIONAL, Rockville, MD, USA (https://www.aoac.org/aoac_prod_imis/AOAC_Docs/SMPRs/SMPR%202016_002.pdf).

Supporting bibliography for validation of antibodies and extraction methods of food samples:
Segura-Gil et al. (2020). Influence of different extraction conditions on the detection of glycinin and β-conglycinin in model processed foods by ELISA. Food Additives and Contaminats Part A.
Segura-Gil et al. (2019). Development of sandwich and competitive ELISA formats to determine β-conglycinin: Evaluation of their performance to detect soy in processed food. Food Control (103) 78-85.

 

Legislation:

Reglamento (UE) No 37/2010

Related articles

SKU: ZE/PR/S Categories: ,

How can we help?

Leave us your details. We will contact you.