MICROCYSTEST KIT - Detection of microcystins in drinnking and recreational water

Microcystins are a group of hepatotoxins produced by cyanobacteria during and after blooms in water bodies as a result of eutrophication. They are considered the most harmful cyanotoxins due to their natural abundant and potent toxicity, posing a hazard to animals and humans. Monitoring of drinking and environmental water is therefore very important; and so a provisional upper limit of 1 µg/L in drinking water was introduced by the World Health Organization as a guideline.

Microcystins are a group of hepatotoxins produced by cyanobacteria during and after blooms in water bodies. Consumption of contaminated water with these toxins can promote cancer. The toxicity of microcystins is associated with the inhibition of protein phosphatases 1 and 2A enzymes (PP1 &PP2A), which leads to hepatocyte dysfunction. MicroCystest is the first and only commercially available kit based on the inhibition of the PP2A activity by microcystins, and therefore able to detect all potential toxic microcystins present in the sample (over 120 different varieties of MCs have been described up-to-date).

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MicroCystest is a simple and rapid enzymatic test for the detection of microcystins and nodularins in drinnking and recreational water. The toxicity of microcystins is associated with the inhibition of protein phosphatases 1 and 2A (PP1 and PP2A), which can lead to hepatocyte diysfuntion. MicroCystest kit uses this inhibitory reaction for determination of microcystins concentration by means of a microtiter plate and a colorimetric substrate. The enzyme hydrolyses the substrate and the product can be determined by an absorbance measurement at 405 nm. As the ability of the PP to hydrolyse the substrate depends on the presence of microcystins in the samples, the toxin concentration can be calculated by using a standard curve.

MicroCystest is the first and only commercially available kit based on the inhibition of the PP2A activity by microcystins, and therefore able to detect all potential toxic microcystins present in the sample (over 80 different varieties of MCs have been described up-to-date). The U.S. Environmental Protection Agency (EPA) has verified the performance of MicroCystest kit for detection of microcystins under the Environmental Verification Technology Program (ETV).

Detection of microcystins in water samples
    • Fast: 30 minutesmicrocystest-kit
    • Simple: one step assay
    • Quantitative: working range 0.25-2.5 µg/L
    • Semi-Quantitative: working range 0.5-2.5 µg/L
    • Formats: Plate (48 or 96 wells) and tube
    • Suitable for: drinking and surfece water
    • In Vitro Bioassay: able to detect ALL toxics microcystins
Assay procedure

assay-procedure-detection-of-microcystins-in-water-microcystest

Ring Trials & Publications
    • 2007 & 2008: Ring Trial organized by Universitat Politecnica de Catalunya.Ensayos-Intercolaborativos--Publicaciones
    • 2009, 2010 & 2011: Ring Trial Labaqua-Calitax ( circuito ielab)
    • Microcystest plate has beenVerified in the “Environmental Technology Verification Program” under cooperation and agreement of USEnvironmental Protection Agency (EPA)

 

Publications:

  • Sevilla E., Smienk H., Razquin P., Mata L., Peleato, M.  “Optimization of intracellular microcystin-LR extration of its analysis by protein phosphatase inhibitaion assay” WATER SICIENCE TECHOLOGY, 2009, 60.7, 1903-1909. Smienk H.,
  • Sevilla E., Peleato M., Razquin P., Mata L. “Validación de un kit para la detección de microcistinas en agua” ALIMENTARIA, 2007, Julio/Agost, 104-111. 
  • Sevilla E., Smienk H., Calvin V. M., Razquin P., Peleato M., Mata L., “Aplicación de un método de extracción de microcistinas para ensayos de inhibición enzimática” TECNOLOGÍA DEL AGUA, 2009, Mayo, 38-46

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